Background: Tuberculosis is the leading cause of death due to bacterial infections worldwide, mainly caused by\nMycobacterium tuberculosis. The antigen 85 complex comprises a set of major secreted proteins of M. tuberculosis,\nwhich are potential biomarkers for diagnostic.\nResults: In this work, the first human single chain fragment variable (scFv) antibodies specific for the tuberculosis\nbiomarker 85 B were selected by phage display from na�¯ve antibody gene libraries (HAL7/8). Produced as scFv-Fc\nin mammalian cells, these antibodies were further characterized and analysed for specificity and applicability in\ndifferent tuberculosis antigen detection assays. Sandwich detection of recombinant 85 B was successful in\nenzyme linked immunosorbent assay (ELISA), lateral flow immunoassay and immunoblot. Whereas detection of\nM. tuberculosis cell extracts and culture filtrates was only possible in direct ELISA and immunoblot assays. It was\nfound that the conformation of 85 B, depending on sample treatment, influenced antigen detection.\nConclusions: Recombinant antibodies, selected by phage display, may be applicable for 85 B detection in various\nassays. These antibodies are candidates for the development of future point of care tuberculosis diagnostic kits. Using\n85 B as a biomarker, the antigen conformation influenced by sample treatment is important.
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